Performance Assessment of Routing Protocols for IoT/6LoWPAN Networks

The Internet of Things (IoT) proposes a disruptive communication paradigm that allows smart objects to exchange data among themselves to reach a common goal. IoT application scenarios are multiple and can range from a simple smart home lighting system to fully controlled automated manufacturing chains. In the majority of IoT deployments, things are equipped with small devices that can suffer from severe hardware and energy restrictions that are responsible for performing data processing and wireless communication tasks. Thus, due to their features, communication networks that are used by these devices are generally categorized as Low Power and Lossy Networks (LLNs). The considerable variation in IoT applications represents a critical issue to LLN networks, which should offer support to different requirements as well as keeping reasonable quality-of-service (QoS) levels. Based on this challenge, routing protocols represent a key issue in IoT scenarios deployment. Routing protocols are responsible for creating paths among devices and their interactions. Hence, network performance and features are highly dependent on protocol behavior. Also, based on the adopted protocol, the support for some specific requirements of IoT applications may or may not be provided. Thus, a routing protocol should be projected to attend the needs of the applications considering the limitations of the device that will execute them. Looking to attend the demand of routing protocols for LLNs and, consequently, for IoT networks, the Internet Engineering Task Force (IETF) has designed and standardized the IPv6 Routing Protocol for Low Power and Lossy Networks (RPL). This protocol, although being robust and offering features to fulfill the need of several applications, still presents several faults and weaknesses (mainly related to its high complexity and memory requirement), which limits its adoption in IoT scenarios. An alternative to RPL, the Lightweight On-demand Ad Hoc Distancevector Routing Protocol – Next Generation (LOADng) has emerged as a less complicated routing solution for LLNs. However, the cost of its simplicity is paid for with the absence of adequate support for a critical set of features required for many IoT environments. Thus, based on the challenging open issues related to routing in IoT networks, this thesis aims to study and propose contributions to better attend the network requirements of IoT scenarios. A comprehensive survey, reviewing state-of-the-art routing protocols adopted for IoT, identified the strengths and weaknesses of current solutions available in the literature. Based on the identified limitations, a set of improvements is designed to overcome these issues and enhance IoT network performance. The novel solutions are proposed to include reliable and efficient support to attend the needs of IoT applications, such as mobility, heterogeneity, and different traffic patterns. Moreover, mechanisms to improve the network performance in IoT scenarios, which integrate devices with different communication technologies, are introduced. The studies conducted to assess the performance of the proposed solutions showed the high potential of the proposed solutions. When the approaches presented in this thesis were compared with others available in the literature, they presented very promising results considering the metrics related to the Quality of Service (QoS), network and energy efficiency, and memory usage as well as adding new features to the base protocols. Hence, it is believed that the proposed improvements contribute to the state-of-the-art of routing solutions for IoT networks, increasing the performance and adoption of enhanced protocols.A Internet das Coisas, do inglês Internet of Things (IoT), propõe um paradigma de comunicação disruptivo para possibilitar que dispositivos, que podem ser dotados de comportamentos autónomos ou inteligentes, troquem dados entre eles buscando alcançar um objetivo comum. Os cenários de aplicação do IoT são muito variados e podem abranger desde um simples sistema de iluminação para casa até o controle total de uma linha de produção industrial. Na maioria das instalações IoT, as “coisas” são equipadas com um pequeno dispositivo, responsável por realizar as tarefas de comunicação e processamento de dados, que pode sofrer com severas restrições de hardware e energia. Assim, devido às suas características, a rede de comunicação criada por esses dispositivos é geralmente categorizada como uma Low Power and Lossy Network (LLN). A grande variedade de cenários IoT representam uma questão crucial para as LLNs, que devem oferecer suporte aos diferentes requisitos das aplicações, além de manter níveis de qualidade de serviço, do inglês Quality of Service (QoS), adequados. Baseado neste desafio, os protocolos de encaminhamento constituem um aspecto chave na implementação de cenários IoT. Os protocolos de encaminhamento são responsáveis por criar os caminhos entre os dispositivos e permitir suas interações. Assim, o desempenho e as características da rede são altamente dependentes do comportamento destes protocolos. Adicionalmente, com base no protocolo adotado, o suporte a alguns requisitos específicos das aplicações de IoT podem ou não ser fornecidos. Portanto, estes protocolos devem ser projetados para atender as necessidades das aplicações assim como considerando as limitações do hardware no qual serão executados. Procurando atender às necessidades dos protocolos de encaminhamento em LLNs e, consequentemente, das redes IoT, a Internet Engineering Task Force (IETF) desenvolveu e padronizou o IPv6 Routing Protocol for Low Power and Lossy Networks (RPL). O protocolo, embora seja robusto e ofereça recursos para atender às necessidades de diferentes aplicações, apresenta algumas falhas e fraquezas (principalmente relacionadas com a sua alta complexidade e necessidade de memória) que limitam sua adoção em cenários IoT. Em alternativa ao RPL, o Lightweight On-demand Ad hoc Distance-vector Routing Protocol – Next Generation (LOADng) emergiu como uma solução de encaminhamento menos complexa para as LLNs. Contudo, o preço da simplicidade é pago com a falta de suporte adequado para um conjunto de recursos essenciais necessários em muitos ambientes IoT. Assim, inspirado pelas desafiadoras questões ainda em aberto relacionadas com o encaminhamento em redes IoT, esta tese tem como objetivo estudar e propor contribuições para melhor atender os requisitos de rede em cenários IoT. Uma profunda e abrangente revisão do estado da arte sobre os protocolos de encaminhamento adotados em IoT identificou os pontos fortes e limitações das soluções atuais. Com base nas debilidades encontradas, um conjunto de soluções de melhoria é proposto para superar carências existentes e melhorar o desempenho das redes IoT. As novas soluções são propostas para incluir um suporte confiável e eficiente capaz atender às necessidades das aplicações IoT relacionadas com suporte à mobilidade, heterogeneidade dos dispositivos e diferentes padrões de tráfego. Além disso, são introduzidos mecanismos para melhorar o desempenho da rede em cenários IoT que integram dispositivos com diferentes tecnologias de comunicação. Os vários estudos realizados para mensurar o desempenho das soluções propostas mostraram o grande potencial do conjunto de melhorias introduzidas. Quando comparadas com outras abordagens existentes na literatura, as soluções propostas nesta tese demonstraram um aumento do desempenho consistente para métricas relacionadas a qualidade de serviço, uso de memória, eficiência energética e de rede, além de adicionar novas funcionalidades aos protocolos base. Portanto, acredita-se que as melhorias propostas contribuiem para o avanço do estado da arte em soluções de encaminhamento para redes IoT e aumentar a adoção e utilização dos protocolos estudados

LC-MS Analysis and Antifungal Activity of <i>Turnera subulata</i> Sm.

Fungi of the Candida genus are responsible for invasive candidiasis, which affects people all over the world and has high mortality rates. This is due to their virulence factors, which give them great resistance and pathogenicity. In addition, the emergence of multidrug-resistant strains makes it difficult to treat these infections. In this way, natural products have emerged as an alternative to standard drugs, where plants known for their medicinal properties such as Turnera subulata become attractive to research. The present work aimed to analyze the ethanol extract of Turnera subulata leaves against standard strains of Candida albicans, Candida krusei and Candida tropicalis using broth microdilution techniques. The identification of the compounds in T. subulata leaves by LC-MS revealed the presence of a wide variety of substances such as carboxylic acids and terpenes, with flavonoids and fatty acids being more evident. The antifungal assays showed that the extract was not able to inhibit the growth of the tested strains at concentrations with a clinical relevance. However, at higher concentrations, it was able to inhibit the fungal dimorphism of C. albicans and C. tropicalis. It is possible that the T. subulata extract has potential as an inhibitor of fungal virulence factors without affecting the cell viability. Further research should be carried out in order to assess its inhibitory potential for other fungal virulence factors

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora